Abstract
The aim of this study was to examine the effects of chlorogenic acid (CGA) on glucose and lipid metabolism in late diabetic db/db mice, as well as on adiponectin receptors and their signaling molecules, to provide evidence for CGA in the prevention of type 2 diabetes. We randomly divided 16 female db/db mice into db/db-CGA and db/db-control (CON) groups equally; db/m mice were used as control mice. The mice in both the db/db-CGA and db/m-CGA groups were administered 80 mg/kg/d CGA by lavage for 12 weeks, whereas the mice in both CON groups were given equal volumes of phosphate-buffered saline (PBS) by lavage. At the end of the intervention, we assessed body fat and the parameters of glucose and lipid metabolism in the plasma, liver and skeletal muscle tissues as well as the levels of aldose reductase (AR) and transforming growth factor-β1 (TGF-β1) in the kidneys and measured adiponectin receptors and the protein expression of their signaling molecules in liver and muscle tissues. After 12 weeks of intervention, compared with the db/db-CON group, the percentage of body fat, fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c) in the db/db-CGA group were all significantly decreased; TGF-β1 protein expression and AR activity in the kidney were both decreased; and the adiponectin level in visceral adipose was increased. The protein expression of adiponectin receptors (ADPNRs), the phosphorylation of AMP-activated protein kinase (AMPK) in the liver and muscle, and the mRNA and protein levels of peroxisome proliferator-activated receptor alpha (PPAR-α) in the liver were all significantly greater. CGA could lower the levels of fasting plasma glucose and HbA1c during late diabetes and improve kidney fibrosis to some extent through the modulation of adiponectin receptor signaling pathways in db/db mice.
Highlights
At the end of the experiment, compared with the db/db-CON group, the visceral adipose tissue (VA) percentage in db/db-chlorogenic acid (CGA) was significantly reduced by 10% (P
In db/m mice, Lee's index and the HbA1c level in the CGA group were significantly decreased by 42% and 45.2%, respectively (P
We used db/db mice as a type 2 diabetic mouse model to observe the impact of CGA on glucose and lipid metabolism disorders and diabetic nephropathy
Summary
In 2011, there were 366 million people with diabetes, and this number is expected to rise to 552 million by 2030 [1]. In China, the age-standardized prevalence of total diabetes and prediabetes was 9.7%, amounting to 92.4 million Chinese adults with diabetes, and this disease has already become a major public health problem [2]. Chlorogenic Acid Improves Late Diabetes in db/db Mice. Type 2 diabetes and its complications are a serious hazard to human health. As the occurrence and development of type 2 diabetes are closely related to unhealthy lifestyles (such as unreasonable dietary structure and limited physical activity), finding natural bio-active ingredients in frequently consumed foods to attenuate diabetes has become urgent and significant
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