Abstract
Blending is one of the effective approaches in preparing tailored materials with a wide range of properties. Thus, chitosan-based polymers have been fabricated and used as wound dressings since they possess better properties than those of the constituent materials. The objective of this work was to evaluate the biocompatibility and biodegradability of biodegradable blend films based on polyethylene glycol-co-fumarate (PEGF) and chitosan (Ch). The blend films of Ch/PEGF were prepared by solution casting/solvent evaporation method. Degradation behavior of these blend films was evaluated in a simulated fluid at physiological pH supplemented with lysozyme at a concentration similar to that in human serum by weight loss of the films and changes in the pH of media. When the pH of incubation media was analyzed, with an increase of PEGF content in the blend films, the degradation rate increased accordingly. The pH of the media of samples was not significantly changed at any measured time point and all films kept their integrities during 28 days. The biocompatibility of the films and cell behavior on the surface of these films were investigated by in vitro tests. Biological assessment using mouse fibroblast cell line L929 on the blend films of Ch/PEGF indicated that films supported the attachment, spreading and proliferation of cells. Since the Ch/PEGF films are biocompatible with the tailored biodegradation rate, they might have a great prospective position in the application of wound dressings.
Highlights
The degradation rate of a wound dressing film has to match the rate of wound healing during the healing process (Cao and Wang 2009)
Biodegradation behavior was characterized at physiological pH and enzyme concentration similar to that in human serum
Similar to blends based on chitosan and PEG, the results showed that blends of Ch/polyethylene glycol-co-fumarate (PEGF) could improve cell adhesion (Zhang et al 2002)
Summary
The degradation rate of a wound dressing film has to match the rate of wound healing during the healing process (Cao and Wang 2009). Chitosan (Ch) is a biocompatible, biodegradable, non-toxic and antibacterial biopolymer that has been well known as being able to accelerate the healing process of wounds in humans (Mir. Progress in Biomaterials (2018) 7:143–150 activity, permeability and fluid absorption (Hashemi Doulabi et al 2013b, 2015). Motivated by our preliminary results (Hashemi Doulabi et al 2013b), the main goal of this research was to investigate in vitro degradability, biocompatibility and cytocompatibility of Ch/PEGF blend films as important requirements for a wound dressing material. Their biodegradation profile and in vitro cell behavior response confirmed their potential for medical applications. To the best of our knowledge, long-term in vitro degradation studies of the Ch/PEGF blend film at simulated physiological conditions, i.e., pH, enzyme, etc., as well as cell behavior evaluations have not yet been published
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