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Abstract
This study aimed at investigating the chitinase enzyme activity produced by chitinolytic bacteria from the skin of blue swimmer crab (Portunus pelagicus) and identification of the genus isolate. This study consists of two stages: firstly, the qualitative and quantitative activity of the chitinase enzyme; and secondly, biochemical identification of the bacteria. The quantitative chitinase enzyme activity is measured using the UV-Vis spectrophotometer UV-Vis at the wavelength at 660 nm. The chitinase enzyme is obtained from the isolation of chitinolytic bacteria cultured within a media to grow solid chitin, which contains colloidal chitin substrate as chitinase inductor at the temperature of 30°C. The highest chitinolytic activity is obtained from the 24 h supernatant culture, with a value of enzyme activity at 0.149 U/mL. Macroscopic and microscopic identification showed that the chitinolytic bacteria isolate R1, whereas the biochemical cell shows the characteristics of the genus Pseudomonas. Keywords: biodegradable, chitinase, spectrophotometer, Portunus pelagicus, Pseudomonas DOI: 10.25165/j.ijabe.20211403.5273 Citation: Sulistijowati R, Sudin, Harmain R M. Chitinase activity potential and identification of chitinolytic bacteria isolated of swimmer crab’s cell. Int J Agric & Biol Eng, 2021; 14(3): 228–231.
Highlights
The earth chitin is among the most abundant biomass present
This study aimed at investigating the chitinase enzyme activity produced by chitinolytic bacteria from the skin of blue swimmer crab (Portunus pelagicus) and identification of the genus isolate
Chitinase enzyme produced by chitinolytic bacteria has the potential to degrade chitin due to the existence of the chitinase enzyme, which enables the conversion of abundantly available chitin into usable products
Summary
The earth chitin is among the most abundant biomass present. Chitinase plays an important role in the decomposition of chitin and potentially in the utilization of chitin as a renewable resource. Types of an enzyme that degraded the chitin are chitinase enzyme[1]. Chitinase enzyme produced by chitinolytic bacteria has the potential to degrade chitin due to the existence of the chitinase enzyme, which enables the conversion of abundantly available chitin into usable products. The bacteria that produce chitinase enzyme or chitinolytic bacteria can be found within the habitat that contains a high level of chitin, such as in the cell of the blue swimmer crab. The enzymatic method uses enzymes or bacteria for deproteinization by adding enzyme or by the involvement of chitinase to degrade chitin. The production of chitinase enzyme from Aspergillus niger utilized the blue swimmer crab’s waste as inducer[4]. Chitinase activity of isolates chitinolytic bacteria can degrade different chitins
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