Abstract
High-performance liquid chromatography with a chiral crown ether stationary phase and capillary electrophoresis (CE) with a chiral crown ether dissolved in the operating buffer were used for the separation of enantiomers of analogues of DOPA and tyrosine and some analogues of γ-aminobutyric acid (GABA). CE and HPLC yielded similar results for the DOPA and tyrosine analogues. However, for the analogues of GABA, three of the four compounds tested were well resolved by HPLC but only one was well resolved by CE. It was necessary to use an indirect detection scheme for the CE of GABA analogues. The influence of substituents on the different compounds on the resolution factors observed by the two methods is discussed, in addition to the advantages and disadvantages of the two methods in practical applications.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
