Abstract
Chinese liquor is obtained from various grains by fermentation and complex processes. Chinese liquor contains complex ingredients. However, the low contents and presence of ethanol restricted the flavor substances function study. In current study, a flavor substance, homofuraneol (HOMO) was isolated from the Chinese liquor and the potency against H2O2‐induced oxidative damage in HepG2 cells and lifespan‐extending ability in Caenorhabditis elegans were explored. Results indicated that HOMO increased the HepG2 cells cytoactive by eliminating excessive intracellular free radicals, upregulating antioxidant enzyme activity and inhibiting the phosphorylation of mitogen‐activated protein kinases (MAPKs) pathway. Further study revealed that HOMO extended the lifespan of N2 nematodes under normal and oxidative stress conditions. Moreover, RT‐PCR results showed that paraquat activated the expression of PMK‐1 and SKN‐1 was significantly regulated by HOMO. Of note, our results indicated that HOMO recovered the redox states of HepG2 cells by targeting MAPKs and upregulating the stress resistance of nematodes by modulating the expression of stress‐responsive genes, such as DAF‐16.
Highlights
A variety of damaging processes and diseases, such as carcinogenesis, inflammation, aging, and atherosclerosis are related to overproduction of reactive oxygen species (ROS)
We explore the protect potency of the flavor compounds in Chinese liquor against oxidative stress in HepG2 cells and the lifespan-extending functions in C. elegans
The cytotoxicity of HOMO to HepG2 cells was detected by MTT assay, and the results showed that the HOMO performed no cytotoxicity to the HepG2 cells upto 0.180 mg/ml
Summary
A variety of damaging processes and diseases, such as carcinogenesis, inflammation, aging, and atherosclerosis are related to overproduction of reactive oxygen species (ROS). As reported, increased oxidative stress may damage macromolecules by triggering mitogen-activated protein kinase (MAPK) signaling pathways (Xiong, Dong, Wang, Tong, & Li, 2015). | 3165 elegans is a famous model for studying aging with short lifespan, rapid generation, and experimental flexibility (David et al, 2010; Oh & Kim, 2013). It is widely used in biomedical and toxicological researches (Jia et al, 2017; Wei, Zeng, Ke, & Wang, 2016). We explore the protect potency of the flavor compounds in Chinese liquor against oxidative stress in HepG2 cells and the lifespan-extending functions in C. elegans
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