Chilling tolerance of potato plants transformed with a yeast-derived invertase gene under the control of the B33 patatin promoter

Abstract

Tolerance to chilling was compared under in vitro conditions in potato plants ( Solanum tuberosum L., cv. Desiree) transformed with a yeast-derived invertase gene under the control of the B33 class 1 tuber-spe- cific promoter (the B33-inv plants) and potato plants transformed only with a reporter gene (the control plants). The expression of the inserted yeast invertase gene was proved by following the acid and alkaline invertase activities and sugar contents in the leaves under the optimum temperature (22 ° C). The total activities of acid and alkaline invertases in the B33-inv plants exceeded those in the control plants by the factors of 2-3 and 1.3, respectively. In the B33-inv plants, the activity of acid invertase twice exceeded that of the alkaline invertase, whereas the difference equaled 12% in the control plants. The contents of sucrose and glucose increased in the B33-inv plants by 21 and 13%, respectively, as compared to the control. Chilling at +3 and -1 ° C for 1, 3, and 6 h did not affect the rate of lipid peroxidation, as measured by the content of malonic dialdehyde (MDA) in the leaves of the genotypes under study. Only the longer exposures (24 h at +3 and -1 ° C and 7 days at +5 ° C) produced a significant decline in the MDA content in the B33-inv plants, as compared to the control. Following short freezing (20 min at -9 ° C), the content of MDA increased by 50% in the leaves of the control plants, while in the B33-inv plants, cold-treated and control plants did not differ in the MDA content. The authors presume that the potato plants transformed with the yeast invertase gene acquire a higher tolerance to low temperatures as compared to the control plants, apparently due to the changes in sugar ratio produced by the foreign invertase.