Chest staining variation as a signal of testosterone levels in male Verreaux's Sifaka

Abstract

Male Verreaux's sifaka ( Propithecus verreauxi) exhibit variation in the staining of chest hair in association with the activity of the sternal gland. Scent-marking behavior and social relationships have been shown to vary with the state of chest staining. Research on other mammals suggests that sternal gland activity is modulated by testosterone. The goal of this study was to examine the relationship among fecal testosterone, testes mass, and chest staining in sifaka. I predicted that for sifaka (1) testosterone and testes mass are positively correlated, (2) stained males have higher testosterone and larger testes than clean-chested males, (3) testes mass is greater during the mating season than the birth season, and (4) testes mass is comparable to species with multimale mating systems. Eighty fecal samples were collected from eight adult males in five social groups in the Kirindy Mitea National Park of Madagascar during the mating season and analyzed using enzyme immunoassays. Males were captured during the mating season and the subsequent birth season to measure body mass and testes size and to document chest staining. Stained males had significantly higher fT and larger testes mass than clean-chested males. Testes mass was significantly greater during the mating season than the birth season for all males. However, the stained males exhibited less testes mass reduction during birth season than clean-chested males. These results suggest that the activity of the sternal gland is regulated by testosterone and that sexual selection has shaped male morphological variation in Verreaux's sifaka.