Chemiluminescence method for the quantitative determination of vitamin B1 and its phosphate esters in pharmaceutical and biological samples

Abstract

A large number of methods have been proposed for the quantitative determination of vitamin BI (thiamine): gravimetric [i], fluorometric [2, 3], titrimetric [3], spectrophotometric [4], polarographic [3, 5], and several others [6, 7]. The common deficiency of these methods (apart from fluorometry) is their inadequate specificity. The determination of vitamin B: and its phosphate esters by these methods suffers from interference by various organic and inorganic substances, particularly the products of hydrolysis (thiazole and aminopyrimidine derivatives) and oxidation (thiochrome and thiamine disulfide) of the original substances [8]. This necessitates prolonged and difficult operations for the preliminary separation of thiamine from the impurities by, for example, extraction, adsorption, and chromatographic methods [2, 3, 9-11]. Furthermore the bound forms of natural vitamin BI, such as cocarboxylase, require enzymolysis to thiamine before fluorometric determination [3]. The accuracy of the extant methods for determining vitamin BI varies from 0.08 [6] to 20% [2], depending on the nature of the test sample. The search for rapid, simple, and reliable methods continues to be urgent.