Abstract

AbstractA chemiluminescence (CL) phenomenon was observed when gossypol was injected into a reaction mixture of N‐bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF). Based on this phenomenon, a rapid and sensitive method for the determination of gossypol was established. Under the optimum conditions, the linear range was from 1.0 × 10−9 to 1.0 × 10−6 M. The detection limit was 1.0 × 10−10 M. The method has been applied to the determination of gossypol in cottonseed, cottonseed oil, pharmaceutical and biological fluids with satisfactory results. The possible CL reaction mechanism was discussed briefly.

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