Abstract
A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.
Highlights
Over the last decade, biochemical interactions between non-thermal plasmas (NTPs) and living objects have steadily gained increased attention due to recent developments in plasma medicine[1]
In order to answer such important questions, we carefully examined and compared the effects of two types of non-thermal plasmas and ozone, exerted on living cells
Fourier transform infrared (FTIR) transmittance spectra gave an overview of the composition of the air and helium (He) NTPs analyzed in the jet (Fig. 1a)
Summary
Biochemical interactions between non-thermal plasmas (NTPs) and living objects have steadily gained increased attention due to recent developments in plasma medicine[1]. The exact molecular mechanisms of how NTP changes cellular functionality are not known It is believed, that the main biological consequences of cell treatment with NTP are intracellular RNS and ROS appearance[10,11,12]. The aim of this study is to investigate and compare the effects of two chemically different NTPs and ozone, on physiological and pathophysiological cellular functions. Bearing in mind the discrepancies in literature concerning cell death pathways triggered by NTP, we hypothesized that the chemical composition of NTP would significantly affect which signaling pathway will be activated by distinct NTPs. This study gathers information on the potential molecular targets of different non-thermal plasmas and provides tentative molecular mechanisms of NTPs action on living cells
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