Abstract
The hydrodistillation of the fresh leaves of Hyptis spicigera gave a colourless volatile oil with yield of 0.65%.The volatile oil gave forty compounds on the basis of gas chromatography-mass spectroscopy (GC-MS) with low composition of cineole(4.11%) and caryophyllene (2.61%) while α-pinene (12.16%) β-pinene(9.47%) and α-phellandrene (10.19%) were predominant compounds. The biological activity of the volatile oils was evaluated in vitro for activity against Trypanosoma brucei brucei (Tbb) and was found to possess anti-trypanosoma activity in vitro in a dose dependent pattern at 0.5 µg/ml in 6 min. This activity showed the volatile oils from H. spicigera leaves to be a potential trypanocide. Key words: Hyptis spicigera, gas chromatography-mass spectroscopy,Trypanosoma brucei brucei, trypanosome, volatile oils.
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