Charge transfer of ZnTPP/C60 cocrystal-hybridized bioimplants satisfies osteosarcoma eradication with antitumoral, antibacterial and osteogenic performances

Abstract

Inferior bone-tissue regeneration, rapid tumor recurrence, and severe bacterial infection are the dominating challenges of osteosarcoma resection, which result in the failure of surgical intervention, and even amputation in serious circumstances. Herein, the multifunctional scaffolds (termed as ZCBS) are elaborately designed by in-situ integration of two-dimensional (2D) cocrystals with bioactive-glass scaffolds (BS) for significant osteosarcoma ablation, bacterial sterilization, and neo-bone regeneration. The efficient charge transfer between electron donor (zinc (II) tetraphenylporphyrin, ZnTPP) and acceptor (fullerene, C60) molecules of 2D cocrystals facilitates ZCBS with prominent photothermal-conversion performance for osteosarcoma elimination and bacterial eradication. Meanwhile, the gradual release of C60 and Zn2+ is beneficial to the reinforced antibacterial capability of ZCBS. Furthermore, the macropore structures and progressive release of Zn2+, Ca2+, and PO43- endow ZCBS with excellent osteogenic performance in promoting bone-tissue regeneration. Collectively, the engineered ZCBS bioscaffolds feature intrinsically multifunctional properties including antitumor, antibacterial, and osteogenic bioactivities, which support ZCBS for efficient osteosarcoma eradication, antibacterial treatment, and bone-tissue regeneration both in vitro and in vivo. Therefore, the developed “cocrystal engineering” strategy towards ZCBS with multifunctional bioactivities offer a competitive approach for osteosarcoma- and bacterial infection-induced bone regeneration.