Abstract
A method is described that enables separation of complex triglyceride mixtures like those occurring in blends of vegetable fats, animal depot fats and serum lipids. By combination of adsorption chromatography on AgNO3-impregnated silicagel minicolumns and subsequent HPLC four characteristic triglyceride patterns can be obtained for each sample. The separation achieved reflects the degree of saturation and the chain length of fatty acids occurring in the triglyceride mixture. The isocratic HPLC is carried out with Hypersil ODS (5 μm) using propionitrile as eluent. The substances are detected with a sensitive differential-refractometer. 1 ml serum or 500 μg fat or oil are sufficient for a full description of the triglyceride patterns. Using this procedure 56 triglycerides could be characterized or identified by determination of their ECN-,t rel-values and their fatty acid moieties. The method is applicable for the detection of adulteration, blending and hydrogenation of fats and oils and can be used especially in physiological research due to its low sample need.
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