Characterization of two different Ca2+ uptake and IP3-sensitive Ca2+ release mechanisms in microsomal Ca2+ pools of rat pancreatic acinar cells

Abstract

We have examined the effect of the Ca2+ (Mg2+)-ATPase inhibitors thapsigargin (TG) and vanadate on ATP-dependent 45Ca2+ uptake into IP3-sensitive Ca2+ pools in isolated microsomes from rat pancreatic acinar cells. The inhibitory effect of TG was biphasic. About 40-50% of total Ca2+ uptake was inhibited by TG up to 10 nM (apparent Ki approximately 4.2 nM, Ca2+ pool I). An additional increase of inhibition up to 85-90% of total Ca2+ uptake could be achieved at 15 to 20 nM of TG (apparent Ki approximately 12.1 nM, Ca2+ pool II). The rest was due to TG-insensitive contaminating plasma membranes and could be inhibited by vanadate (apparent Ki approximately 10 microM). In the absence of TG, increasing concentrations of vanadate also showed two phases of inhibition of microsomal Ca2+ uptake. About 30-40% of total Ca2+ uptake was inhibited by 100 microM of vanadate (apparent Ki approximately 18 microM, Ca2+ pool II). The remaining 60-70% could be inhibited either by vanadate at concentrations up to 1 mM (apparent Ki approximately 300 microM) or by TG up to 10 nM (Ca2+ pool I). The amount of IP3-induced Ca2+ release was constant at approximately 25% over a wide range of Ca2+ filling. About 10-20% remained unreleasable by IP3. Reduction of IP3-releasable Ca2+ in the presence of inhibitors showed similar dose-response curves as Ca2+ uptake (apparent Ki approximately 3.0 nM for IP3-induced Ca2+ release as compared to approximately 4.2 nM for Ca2+ uptake at TG up to 10 nM) indicating that the highly TG-sensitive Ca2+ pump fills the IP3-sensitive Ca2+ pool I. At TG concentrations > 10 nM which blocked Ca2+ pool II the apparent Ki values were approximately 11.3 and approximately 12.1 nM, respectively. For inhibition by vanadate up to 100 microM the apparent Ki values were approximately 18 microM for Ca2+ uptake and approximately 7 microM for Ca2+ release (Ca2+ pool II). At vanadate concentrations up to 1 mM the apparent Ki values were approximately 300 and approximately 200 microM, respectively (Ca2+ pool I). Both Ca2+ pools I and II also showed different sensitivities to IP3. Dose-response curves for IP3 in the absence of inhibitors (control) showed an apparent Km value for IP3 at 0.6 microM. In the presence of TG (inhibition of Ca2+ pool I) the curve was shifted to the left with an apparent Km for IP3 at 0.08 microM.(ABSTRACT TRUNCATED AT 400 WORDS)