Abstract
The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and phylogenetic analyses indicated that TGV-[Bi2] constitutes a novel begomovirus species named Tomato yellow spot virus (ToYSV), closely related to Sida mottle virus (SiMoV).
Highlights
The Geminiviridae family is comprised of plant viruses with a circular single-stranded DNA genome, and a particle morphology of twinned incomplete icosahedra (Rojas et al, 2005)
The replicative form (RF) of the viral genome was extracted from the collected plant (Gilbertson et al, 1991), treated with RNase A to eliminate possible viruses occurring in mixed infections, and used for the biolistic inoculation (Aragão et al, 1996) of Nicotiana benthamiana plants
The TGV-[Bi2] isolate was capable of systemically infecting plants of Amaranthaceae (G. globosa) and Solanaceae, including C. annuum 'Ikeda', D. stramonium, L. esculentum 'Miller Early Pack', 'Rutgers' and 'Santa Clara', N. benthamiana, N. clevelandi, N. glutinosa, N. rustica, N. tabacum and N. silvestris (Table 2)
Summary
The Geminiviridae family is comprised of plant viruses with a circular single-stranded DNA genome, and a particle morphology of twinned incomplete icosahedra (Rojas et al, 2005). Viruses classified into the genus Begomovirus are transmitted by the whitefly Bemisia tabaci (Homoptera: Aleyrodidae), infect dicotyledoneous plants, and have mono- or (mostly) bipartite genomes. Novel begomovirus species infecting tomato (Lycopersicon esculentum Mill.) were detected in the 1990’s in different regions of Brazil, after the introduction and quick dissemination of B biotype of B. tabaci (Ribeiro et al, 2003). In the Triângulo Mineiro region of Minas Gerais State, a novel bipartite begomovirus was detected in tomato plants growing under greenhouse conditions (Fernandes et al, 2006). The comparison of the complete nucleotide sequences of the DNA-A and DNA-B
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