Abstract
The establishment and partial characterization of continuous cell lines fromCyprinus carpio is described. Three new cell lines designated CCT, CCG, and CCF, were established from testis, gill, and fin tissue of color carp,C. carpio, respectively. These cell lines have been subcultured more than 500 times since their initiation in May 1984. The optimal incubation temperature for growth is 31° C for CCT and 37° C for CCG and CCF. The modal chromosome numbers for CCT, CCG, and CCF cell lines are 38, 200, and 100, respectively. The duration of the cell cycle at 31° C for each cell line is 10.18 h (CCT), 15.28 h (CCG), and 18.76 h (CCF). All three cell lines are refractory to chum salmon virus. The CCT cell line is susceptible to three serotypes (Ab, Sp, and VR-299) of infectious pancreatic necrosis virus (IPNV), eel virus european, milkfish birnavirus, hard clam birnavirus, and eel herpesvirus from Formosa. In contrast, both CCG and CCF cell lines possess a very strict viral susceptibility. Only the IPNV Sp. serotype was seen to infect those two cell lines in vitro.
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