Abstract
BackgroundCytoplasmic polyadenylation element binding proteins (CPEBs) regulate translation by binding to regulatory motifs of defined mRNA targets. This translational mechanism has been shown to play a critical role in oocyte maturation, early development, and memory formation in the hippocampus. Little is known about the presence or functions of CPEBs in the retina. The purpose of the current study is to investigate the alternative splicing isoforms of a particular CPEB, CPEB3, based on current databases, and to characterize the expression of CPEB3 in the retina.ResultsIn this study, we have characterized CPEB3, whose putative role is to regulate the translation of GluR2 mRNA. We identify the presence of multiple alternative splicing isoforms of CPEB3 transcripts and proteins in the current databases. We report the presence of eight alternative splicing patterns of CPEB3, including a novel one, in the mouse retina. All but one of the patterns appear to be ubiquitous in 13 types of tissue examined. The relative abundance of the patterns in the retina is demonstrated. Experimentally, we show that CPEB3 expression is increased in a time-dependent manner during the course of postnatal development, and CPEB3 is localized mostly in the inner retina, including retinal ganglion cells.ConclusionThe level of CPEB3 was up-regulated in the retina during development. The presence of multiple CPEB3 isoforms indicates remarkable complexity in the regulation and function of CPEB3.
Highlights
Cytoplasmic polyadenylation element binding proteins (CPEBs) regulate translation by binding to regulatory motifs of defined mRNA targets
The current study demonstrates the existence of multiple CPEB3 transcript variants in the retina
One of the variants is selectively expressed among different tissue-types We investigated the expression of CPEB3 mRNA within the P60 mouse retina
Summary
Cytoplasmic polyadenylation element binding proteins (CPEBs) regulate translation by binding to regulatory motifs of defined mRNA targets. This translational mechanism has been shown to play a critical role in oocyte maturation, early development, and memory formation in the hippocampus. Modification of regulatory factors binding to mRNA motifs in the 3' or 5'. Accumulated evidence indicates that mRNA-specific regulatory factors exist as either multi-protein complexes, such as cytoplasmic polyadenylation element binding proteins (CPEBs) [2], or multi-proteins complexes containing a non-coding RNA (siRNA or miRNAs) [3]. Reports on the existence of these translational control mechanisms have added another layer of complexity to our understanding of gene regulation but this has been little explored in the retina
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