Abstract
Mobile DNA elements play a significant evolutionary role by promoting genome plasticity. Insertion sequences are the smallest prokaryotic transposable elements. They are highly diverse elements, and the ability to accurately identify, annotate, and infer the full genomic impact of insertion sequences is lacking. Halanaerobium hydrogeniformans is a haloalkaliphilic bacterium with an abnormally high number of insertion sequences. One family, IS200/IS605, showed several interesting features distinct from other elements in this genome. Twenty-three loci harbor elements of this family in varying stages of decay, from nearly intact to an ends-only sequence. The loci were characterized with respect to two divergent open reading frames (ORF), tnpA and tnpB, and left and right ends of the elements. The tnpB ORF contains two nearly identical insert sequences that suggest recombination between tnpB ORF is occurring. From these results, insertion sequence activity can be inferred, including transposition capability and element interaction.
Highlights
Halanaerobium hydrogeniformans is an anaerobic extremophile isolated in 2007 from Soap Lake, a meromictic haloalkaline lake in eastern Washington State
This study presents a detailed characterization of the IS200/IS605 family members within
Of note were the IS200/IS605, IS607 family members. Manual curation of these elements revealed that they were a single IS200 member, 22 IS605 members, and one PATE-like sequence that was not identified by ISsaga
Summary
Halanaerobium hydrogeniformans is an anaerobic extremophile isolated in 2007 from Soap Lake, a meromictic haloalkaline lake in eastern Washington State. This organism gained attention for its unique metabolic capabilities and potential for industrial applications. Among the 2463 genes annotated in the genome, 72 transposase genes belonging to eight insertion sequence families were originally identified [1]. This put the genome at approximately 3% transposable elements, which is higher than most bacterial genomes [2]. Insertion sequences can be categorized into four groups based on their catalytic mechanisms for transposition. These groups are 1) the DDE, so called for the conserved catalytic DDE motif, 2) Y and
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
