Abstract
The fetus is in a constant anabolic state and responsive to insulin. To examine the possible role of the insulin cell surface receptor in mediating these effects, we have studied insulin-receptor interactions in the developing rat liver. Compared to adult hepatic membranes, specific [125I]iodoinsulin binding was approximately 2 times greater in liver tissues from 21-day-old fetal and 1-day-old neonatal rats. By several criteria (pH, temperature, and time dependencies; specificity; receptor degradation properties; and insulin dissociation characteristics), insulin receptors from perinatal and adult hepatic membranes were similar. At 20 C, however, adult hepatic membranes degraded approximately 3 times as much insulin as liver tissue from 1-day-old neonatal rats. When insulin degradation by adult hepatic membranes was minimized by incubation with N-ethylmaleimide (NEM) or at 4 C, [125I]iodoinsulin binding in perinatal liver membranes remained significantly greater. Scatchard analysis revealed curvilinear pl...
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