Abstract
BackgroundThe aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniae were obtained during routinely diagnostic activities between March 2009 and January 2017.ResultsIsolates were confirmed as S. pneumoniae by bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype.ConclusionsThis study represents the first comprehensive investigation on characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted.
Highlights
The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses
Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) [14] and genotyped by multilocus sequence typing (MLST)
The presence of mutations within fragments of the genes encoding either a dihydropteroate synthase (DHPS) that plays a role in conferring resistance to sulfamethoxazole or a dihydrofolate reductase (DHFR) conferring resistance to trimethoprim in S. pneumoniae were tested by PCR followed by DNA sequence analysis [22]
Summary
The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. Streptococcus pneumoniae is a major human pathogen that colonizes the upper respiratory tract and causes both life-threatening diseases such as pneumonia, sepsis and meningitis and sinusitis and otitis in both children and adults [1]. S. pneumoniae is responsible for community-acquired respiratory tract infections in infants. S. pneumoniae is assumed to be a human pathogen only. Pneumococci can exchange DNA in their natural habitat, the human mouth and throat. This environment is populated by several streptococcal species, which form a ‘gene pool’ out of which the pneumococci can recruit resistance genes.
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