Characterization of sexual size dimorphism and sex-biased genes expression profile in the olive flounder.

Abstract

Sexual size dimorphism (SSD) is a widespread phenomenon in fish species, including in the olive flounder. Although it is well established that female olive flounders acquire more bone mass than males, the underlying mechanism and timing of this SSD remains controversial. Here, the gene expression profiles of adult male and female olive flounder fish were explored to better understand the SSD mechanisms. Using RNA sequencing, a total of 4784 sex-biased differentially expressed genes (DEGs) in the fin with asymptotic growth after maturity were identified, among which growth-related factors were found. Gene ontology and pathway enrichment studies were performed to predict potential SSD-related genes and their functions. According to functional analysis, negative regulation of cell proliferation was significantly enriched in males, and anabolism related genes were highly expressed in females. In addition, pathway analysisusing the Kyoto Encyclopedia of Genes and Genomes database revealed that five sexual dimorphism-related candidate genes (bambia, smurf1, dvl2, cul1a, and dvl3) were enriched in osteogenesis-contributing pathways. These results suggest that these five candidate genes may be relevant for skeletal development in olive flounders. Altogether, this study adds new knowledge for a better understanding of SSD-related growth traits in olive flounder, which can be used for enhancing aquaculture productivity with reduced production costs.