Abstract

Indirect hemagglutination studies were performed on 10 incomplete anti-Rh sera from mothers after incompatible pregnancies, on 19 incomplete anti-Rh plasma samples from preimmunized and repeatedly boosted individuals and on 3 anti-Rh plasma pools. Group O Rh-positive red blood cells were agglutinated by antisera to immunoglobulins. Anti-Rh antibodies after incompatible pregnancies were found to belong to the immunoglobulin class IgG and to the subclasses IgGl, IgG3 and IgG4 only. In most anti-Rh samples from preimmunized and repeatedly boosted subjects, IgG and IgA were present. In six anti-Rh samples of this group, the subclass IgG2 could be demonstrated.

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