Abstract
Abstract Skin-sensitizing antibody with characteristics similar to spontaneously occurring canine and human reagin can be induced in nonatopic humans and dogs by the injection of antigen in emulsion. In this study the response of normal dogs, each injected with emulsified ragweed extract in incomplete Freund's adjuvant, was evaluated by several immunologic techniques. Hemagglutinating and blocking antibodies appeared by the second week, were maximal at the third week, and persisted throughout the study. Skin-sensitizing antibody appeared and was maximal by the fourth week, then gradually declined until it was undetectable by the twentieth week. Antibody demonstrable by co-precipitation appeared by the third week and persisted throughout the study. Radioimmunoelectrophoresis during this time demonstrated antigen binding by two immunoglobulins migrating in the gamma region. No antibody against ragweed could be demonstrated by PCA in guinea pigs. The levels of skin-sensitizing antibody could not be correlated with the titers of antibodies detected by hemagglutination, blocking, or co-precipitation, suggesting that these latter antibodies were separate from the skin-sensitizing antibody.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
