Abstract
Messenger RNA was purified from immature radish seeds, dry seeds and 24-h-old seedlings. It was translated in a rabbit reticulocyte lysate in vitro protein-synthesis system. The three mRNA preparations have an overall similar activity and code for polypeptides ranging from 10 000 to 100 000 daltons. Analysis of the translation products using one- and two-dimensional polyacrylamide gel electrophoresis (PAGE) indicates important changes in the prevalent mRNAs during seed ripening and germination. A subset of mRNA accumulates late in embryogenesis whilst most abundant immature seed mRNA decays. This late mRNA subset and a few other mRNAs detcted in immature seeds constitute the most abundant part of mRNA stored in the seed. Several less abundant mRNAs of the immature seed are also present in the dry seeds. The abundant mRNAs from dry seed disappear during early germination.
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