Abstract
The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.
Highlights
Potato (Solanum tuberosum L.) is one of the most cultivated vegetable crops and it is highly consumed in that country
The objective of this work was to characterize 19 cultivars and eight elite potato clones from the collection maintained by Embrapa Clima Temperado, by using isozyme and protein analysis and RAPD markers
Horizontal electrophoresis of polyacrylamide gels, with 5% or 6%, were used with buffer systems described by Shields et al (1983), and Scandalios (1969)
Summary
Potato (Solanum tuberosum L.) is one of the most cultivated vegetable crops and it is highly consumed in that country. Potato produces the highest yield of energy and proteins per hectare a day (1.4 kg protein ha-1 day-1), overcoming wheat (1.3 kg protein ha-1 day-1) and rice (1.0 kg protein ha-1 day-1). It is a source of vitamins, mainly B and C complexes, and of mineral salts. It is included among high energetic value foods, it presents a low caloric value. It is outstanding for medicinal purpose, such as gastrointestinal and hepatic infections (Silva, 1991)
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