Abstract

Polyphenols from mango puree concentrate were characterized by HPLC with diode array and mass spectrometric detection. After extraction with acetone, further fractionation of polyphenols with ethyl acetate and Sephadex LH-20 was necessary to obtain pure peaks. Five quercetin (Q) glycosides and one kaempferol glycoside were unambiguously identified. The predominant flavonol glycosides were Q 3-galactoside (22.1 mg/kg fresh wt.), Q 3-glucoside (16 mg/kg), and Q 3-arabinoside (5 mg/kg). Among the phenolic acids, gallic acid was predominant (6.9 mg/kg). Quantification of the C-glycoside mangiferin (4.4 mg/kg) was also achieved by the HPLC method described. Using MS/MS, a gallotannin consisting of glucose and four gallic acid units was detected. Due to the presence of both carotenoids and polyphenols, mangos can be considered as an especially rich source of antioxidants.

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