Abstract
Little millet varieties are generally distinguished by morphological descriptors which are being used for seed certification and DUS characterization [1]. But in practical terms, these key differentiation descriptors between varieties of little millet are very fewer and hence difficult to differentiate germplasm accessions. Germplasm registration in NBPGR needs DNA fingerprint to show the uniqueness of germplasm in comparison to existing varieties. DNA fingerprinting is a better option to identify unique markers to differentiate the varieties. Available genomic resources are scarce since little millet is still considered to be an orphan crop. Therefore markers from other cereal genomes such as maize, pearl millet and barnyard millet that are been utilized for DNA fingerprinting purpose with a clue of cereal synteny relationship. Twenty-one morphological descriptors studies revealed that the variety ATL 1 is different from the other varieties for more than 16 morphological characters studied. DNA fingerprinting is attempted in five genotypes of little millets such as BL6, ATL 1, TNPsu 176, Co (Samai) 4, Paiyur 2 using cereal SSR markers. Among the 25 maize SSR markers used two markers viz., phi213984 and phi295450 scored polymorphism by the amplicon size of 310bp and 600bp respectively. From the 25 Pearl millet SSR markers used only one SSR marker found polymorphic at 305bp allele size for ATL 1 and Hence, SSR based DNA fingerprinting helped to differentiate ATL1, the newly released high yielding variety from other genotypes of little millets which can be used for varietal identification purpose.
Highlights
Little millet (Panicum sumatrense) is known as Indian millet
Little millet varieties are generally distinguished by morphological descriptors which are being used for seed certification and DUS characterization [1]
From the 25 Pearl millet SSR markers used only one SSR marker found polymorphic at 305bp allele size for ATL 1 and SSR based DNA fingerprinting helped to differentiate ATL1, the newly released high yielding variety from other genotypes of little millets which can be used for varietal identification purpose
Summary
Little millet (Panicum sumatrense) is known as Indian millet. It is cultivated as a cereal across Nepal, India and Western Myanmar, and its center of origin is West Africa. The wild relative of little millet is P.psilopodium It forms an important role in tribal agriculture in Eastern Ghats of India. The present investigation was conducted to carry out the comprehensive characterization of little millet genotypes based on Distinctness, Uniformity and Stability (DUS) characters for Protection of Plant Varieties and Farmers Rights Authority (PPVFRA) registration because of its property to identify the dissimilarity between the newly released and the existing genotypes as well as to distinguish the germplasm. Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made
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