Abstract
Lipase is a hydrolase enzyme that is widely used in industry. The waters in Mahakam river port become a conducive place for the growth of bacteria that are able to hydrolyze lipids, so that bacteria are thought to be able to produce lipases. This research was conducted to screen bacteria that produced extracellular lipase and characterize the lipase. Screening of lipase-producing bacteria has been done with solid media containing Rodhamin B and the activity of lipase was conducted by titrimetricly. The characterization of isolated bacteria indicated that this bacteria belongs to Gram positive cocci shaped bacteria. Lipase-producing bacteria was successfully selected from the water taken in Mahakam river port Samarinda. The lipase was produced for 36 h. The optimum working conditions of lipase at 40°C and substrate concentration of 6%. Specific activity of bacterial lipase in Mahakam river water at Samarinda Port is 0.7212 U / mg.
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