Abstract
Bacterial disease of silkworm causes significant reduction of silk production leading to huge economic loss. This study aims to isolate bacteria from diseased silkworm and to determine its pathogenicity and antibiotic resistance. A strain of Klebsiella granulomatis has been isolated from silkworm haemolymph which was later identified on the basis of biochemical tests and 16S rRNA gene sequencing. The optimum culture condition of K. granulomatis was determined at pH 7.0 and 37 °C temperature. The strain was resistant to most of the antibiotics used in this study except azithromycin, gentamycin and ciprofloxacin. The strain is capable to reproduce flacherrie like symptoms with high mortality rate when re-injected into healthy silkworm. Treatment with low dose of ciprofloxacin was found to be effective to prevent flacherrie induced by the isolated K. granulomatis strain.
Highlights
Sericulture is an agro-based industry practiced in the greater Rajshahi, Chapai Nawabganj, Natore, Bogra and Naogaon and certain nontraditional areas in Bangladesh
This study aims to isolate bacteria from diseased silkworm and to determine its pathogenicity and antibiotic resistance
A strain of Klebsiella granulomatis has been isolated from silkworm haemolymph which was later identified on the basis of biochemical tests and 16S rRNA gene sequencing
Summary
Sericulture is an agro-based industry practiced in the greater Rajshahi, Chapai Nawabganj, Natore, Bogra and Naogaon and certain nontraditional areas in Bangladesh. It is grouped under village and small enterprises sector that plays major role for the creation of sustainable employment and income (Ishtiaque et al 2013). Livelihood of about 0.1 million people in Rajshahi region is directly or indirectly involved with sericulture industry (Islam et al 2004). The mulberry silkworm, Bombyx mori, has been domesticated for silk production for more than 5,000 years and provides the major source of income for 30 million families. About 34–40 % the total crop in a year has been reported to be loss due to diseases (Sheebha et al 2008)
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