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Abstract
Four nitrate reductase-deficient (NR −) mutants, C25, C27, C32 and C33, were isolated from 100 000 M 2 seedlings of rice ( Oryza sativa L., ssp. indica, cv. IR30) by means of selection for chlorate-resistance. All mutations were monogenic and recessive. Biochemical analysis showed that both NADH- and NADPH-dependent activities of nitrate reductase (NR) in these four mutants were less than 25% of the wild type IR30. However, nitrite reductase activities were more than the wild type. Also, xanthine dehydrogenase (XDH) activities and molybdenum cofactor biosynthesis abilities (MoCo activities) were deficient indicating that these mutants were cnx and not nia type. The cnx mutants could be divided into two groups: one could recover NADH-NR activity by adding 0.5 mM molybdate to the growth medium (C27, C32 and C33) while the other could not (C25). The result of allelism test coincided with the phenotypic classification by adding molybdate. The growth response to tungstate was also investigated in the mutants. None of the mutants classifies to the tungstate-sensitive group as reported in Arabidopsis cnx mutants.
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