Abstract
Introduction:Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.Methods:Here, we report a protocol for the use of a reconfigurable biocompatible azopolymer thin film through a photoinduced reconfigurable structuration of its surface to study nerve growth, differentiation and cell guidance.Results & Discussion:We show that this protocol combined with a spontaneous self-photoinduced polymer is suitable for time-lapse fluorescence video microscopy and can be easily adapted to electron microscopy techniques (SEM) and near-field imaging techniques (AFM).
Highlights
Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.Accepted: November 14, 2018
Cells seeded on this 2D artificial ECM tend to extend following its architecture
fixed cells on photostructurable surfaces prepared with a modified SEM preparation procedure can be visualized by scanning electron microscopy techniques
Summary
Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.Accepted: November 14, 2018. Numerous works and experiments show that cells develop a high sensitivity for chemical and physical features of 2D and 3D substrates used in the goal to control and study their in-vitro proliferation. In this view it has been shown that several components of the artificial Extracellular Matrices (ECM) affect different comportments of cells during growths such as adhesion, proliferation, differentiation, and cell death [1 - 3]. Several signals induced by the material substrate, such as 2D and 3D organization, influence cell’s comportments and highlighted the paramount importance of ECM. The final objectives are aimed to study contact interactions, guidance and mechano-transduction phenomena [2, 4 - 6]
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