Abstract
Aptasensors for detection of thrombin were produced by covalently linking aptamer (ssDNA) to cSWCNT. Those biosensors were fully characterized by cyclic voltammetry to evaluate the electrode surface charge/ligand density. We performed the CV studies of electrostatically bound [Ru(NH3)6]3+ redox markers on aptamer surfaces and calculated aptamer surface charge density from the CV data. Potentiometric detection of thrombin allows then the correlation with the CV results. The electrode surfaces containing higher amount of aptamers exhibited better performance in potentiometric measurements. These investigations will introduce the pathway to build reusable and regenarable aptasensors including a simple, accurate and precise estimation of aptemer surface charge density to characterize the surface and hence to ensure the quality of apatsensors.
Highlights
The immediate detection of biological macro molecules, either pathogens or proteins, is one of the future challenges in medicine and diagnostics [1]
From the Cyclic Voltammetric (CV) experiments we have demonstrated the redox behaviour of [Ru(NH3)6]3+ redox markers on different electrode surfaces and calculated double layer capacitance (CD), redox capacitance (CR), electrode surface area (A) and Ru3+ surface coverage (ГRu)
The capacitance obtained is compared to the reference value for cSWCNT (C* = 10 μF/cm2) [17] so that the surface area is calculated from these equations: AD = CD/C* and ii) AR = CR/C*
Summary
The immediate detection of biological macro molecules, either pathogens or proteins, is one of the future challenges in medicine and diagnostics [1]. The peak current ratio (ipc1/ipa) for cSWCNT/gc and SWCNT-COOR/gc electrode surface complies with the reversible redox process whereas the aptamer-cSWCNT/gc surface exhibits quasi-reversible charge transfer process.
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