Abstract
Diseased bitter gourd plants with severe leaf rolling symptoms were observed in Sichuan province (southwest China). Leaf dips-prepared from symptomatic leaves contained particles ca. 30 nm in diameter. The virus recovered from all samples was identified as Cucumber mosaic virus (CMV) by Western blotting, RT-PCR and sequencing of amplicons. All obtained sequences were identical, and the virus isolate was designated SC-J1. The complete nucleotide sequence of the CP (GenBank accession No. KT932936) of the isolate shared 99% identity to the corresponding regions of strain Hnt (CMV II). Phylogenetic analysis based on the nucleic acid sequence of the CP shows that SC-J1 belongs to CMV subgroup II. To our knowledge, this is the first report of CMV II infecting bitter gourd in China.
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