Abstract
Pseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated infections. The Brazilian isolate, named CCBH4851, is a multidrug-resistant clone belonging to the sequence type 277. The antimicrobial resistance mechanisms of the CCBH4851 strain are associated with the presence of the bla_text {SPM-1} gene, encoding a metallo-beta-lactamase, in combination with other exogenously acquired genes. Whole-genome sequencing studies focusing on emerging pathogens are essential to identify key features of their physiology that may lead to the identification of new targets for therapy. Using both Illumina and PacBio sequencing data, we obtained a single contig representing the CCBH4851 genome with annotated features that were consistent with data reported for the species. However, comparative analysis with other Pseudomonas aeruginosa strains revealed genomic differences regarding virulence factors and regulatory proteins. In addition, we performed phenotypic assays that revealed CCBH4851 is impaired in bacterial motilities and biofilm formation. On the other hand, CCBH4851 genome contained acquired genomic islands that carry transcriptional factors, virulence and antimicrobial resistance-related genes. Presence of single nucleotide polymorphisms in the core genome, mainly those located in resistance-associated genes, suggests that these mutations may also influence the multidrug-resistant behavior of CCBH4851. Overall, characterization of Pseudomonas aeruginosa CCBH4851 complete genome revealed the presence of features that strongly relates to the virulence and antibiotic resistance profile of this important infectious agent.
Highlights
Pseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated infections
Families of Clusters of orthologous groups (COG) were attributed to 5,247 coding sequences (CDSs) comprising 20 functional categories distributed along the chromosome (Fig. 1)
Pseudomonas aeruginosa CCBH4851 is a clinical isolate originating from Brazil, which belongs to the endemic group sequence type 277 (ST-277)
Summary
Pseudomonas aeruginosa is one of the most common pathogens related to healthcare-associated infections. The antimicrobial resistance mechanisms of the CCBH4851 strain are associated with the presence of the blaSPM-1 gene, encoding a metallo-beta-lactamase, in combination with other exogenously acquired genes. Whole-genome sequencing studies focusing on emerging pathogens are essential to identify key features of their physiology that may lead to the identification of new targets for therapy. In 2002, the SPM-1-encoding gene (blaSPM-1 ) was first identified in a clinical isolate recovered from a blood culture of a patient admitted in a hospital located in the state of São Paulo. Thereby, recent whole-genome sequencing studies have focused on emerging MDR P. aeruginosa strains in order to identify key aspects of their physiology that may lead to the identification of new targets for drug development. We believe this study will serve as a useful reference for future research using CCBH4851 as a model organism
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