Abstract
Adult stem cells play an important role in maintaining tissue homeostasis. Although these cells are found in many tissues, the presence of stem cells in the human minor salivary glands is not well explored. Using the explant culture method, we isolated a population of cells with self-renewal and differentiation capacities harboring that reside in the human minor salivary glands, called human minor salivary gland mesenchymal stem cells (hMSGMSCs). These cells show embryonic stem cell and mesenchymal stem cell phenotypes. Our results demonstrate that hMSGMSCs have the potential to undergo mesodermal, ectodermal and endodermal differentiation in conditioned culture systems in vitro. Furthermore, in vivo transplantation of hMSGMSCs into SCID mice after partial hepatectomy shows that hMSGMSCs are able to survive and engraft, characterized by the survival of labeled cells and the expression of the hepatocyte markers AFP and KRT18. These data demonstrate the existence of hMSGMSCs and suggest their potential in cell therapy and regenerative medicine.
Highlights
Adult stem cells play an important role in maintaining tissue homeostasis
An in vivo histone2B green fluorescent protein pulse-chase strategy has been used to define label-retaining cells in the mouse minor salivary glands[19], but far, minimal data have described the presence of mesenchymal stem cells located in human minor salivary glands and little is known about their characteristics
For cells at passage 4, the efficiency of colony formation was 41.00 ± 8.83%, which suggests the existence of a self-renewable population of human minor salivary gland mesenchymal stem cells
Summary
Adult stem cells play an important role in maintaining tissue homeostasis. these cells are found in many tissues, the presence of stem cells in the human minor salivary glands is not well explored. Using the explant culture method, we isolated a population of cells with self-renewal and differentiation capacities harboring that reside in the human minor salivary glands, called human minor salivary gland mesenchymal stem cells (hMSGMSCs). An in vivo histone2B green fluorescent protein pulse-chase strategy has been used to define label-retaining cells in the mouse minor salivary glands[19], but far, minimal data have described the presence of mesenchymal stem cells located in human minor salivary glands and little is known about their characteristics. We report that culture of human minor salivary glands generates a novel mesenchymal stem cell population with self-renewal and multi-lineage differentiation capacities. We demonstrate for the first time that mesenchymal stem cells isolated from human minor salivary glands are able to generate cell repopulation in acute liver injury models, indicating potential multi-organ therapeutic application
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