Abstract
Optimum conditions to maximize the observed resolution and signal-to-noise ratio of the Shimadzu CS-910 scanning densitometer for high-performance thin-layer chromatography (HPTLC) are described. Resolution is shown to depend only on the slit width and is constant for slit widths in the range 0.05–0.80 mm. Signal-to-noise ratios depend on the size of the sampling beam which is fixed by the dimensions of the slit. At small slit dimensions the noise component dominates the signal-to-noise ratio. For scanning HPTLC plates dual-beam single-wavelength operation, linear scanning, scan speeds of 24 or 48 mm min −1, slit widths of 0.5–0.8 mm and slit height ≈ 3.0 mm are recommended for general use.
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