Abstract
Summary Alkylation of rhodopsin in 40 mM cetyltrimethylammonium bromide revealed that the ratio of the light:dark formation of [ 14 C]carbamidomethylated-opsin was 1.0:0.1. A rhodopsin photo-exposed sulfhydryl group was demonstrated in rod outer segment membranes with [ 14 C]parachloromercuribenzoate and [ 14 C]iodoacetamide. Amino acid analysis of native and S-carbamidomethylcysteinyl-opsin, before and after performic acid oxidation, indicated that 1.2–1.3 sulfhydryl groups were available for alkylation per 4.9 total half-cystinyl residues in illuminated rhodopsin.
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