Abstract
Globally, mosquitoes are known to be competent vectors to various arboviruses that cause serious and debilitating diseases to humans and animals. Conversely, mosquitoes harbor a wide array of insect specific viruses (ISVs) that are generally neglected. Extensive characterization of these ISVs is important in understanding their persistence infection effect on host behavior and arbovirus transmission. Herein, we report first time isolation of Tanay virus (TANAV) isolate YN15_103_01 in Anopheles sinensis mosquitoes from Yunnan Province, China. Phylogenetically, the isolate’s nucleotide identity had more than 14.47% variance compared to previous TANAV isolates, and it clustered into an independent branch within the genus Sandewavirus in the newly proposed taxon Negevirus. TANAV growth and high titers was attained in Aag2 cells (107 PFU/mL) but with no CPE observed up to 7 days.p.i. compared to C6/36 cells that exhibited extensive CPE at 48 h.p.i. with titers of 107 PFU/mL. Contrarywise, the viral isolate did not replicate in vertebrate cell lines. Electron microscopy analyses showed that its final maturation process takes place in the cell cytoplasm. Notably, the predicted viral proteins were verified to be corresponding to the obtained SDS-PAGE protein bands. Our findings advance forth new and vital knowledge important in understanding insect specific viruses, especially TANAV.
Highlights
Arthropod-borne viruses cause a significant part of the current global emerging and re-emerging infectious diseases, they pose a huge public health risk worldwide
Adult Anopheles sinensis mosquitoes were trapped from Dehong, Lincang, Pu’er, Xishuangbanna and Honghe, Yunnan province, China, cities bordered Myanmar, Laos and Vietnam
We isolated and characterized Tanay virus-isolate YN15_103_01, from Anopheles sinensis mosquitoes collected in Yunnan province, China
Summary
Arthropod-borne viruses (arboviruses) cause a significant part of the current global emerging and re-emerging infectious diseases, they pose a huge public health risk worldwide. Many ISVs have been described from different mosquito species and majority of these newly discovered ISV’s phylogenetically cluster to similar viral groups associated with classical arboviruses They include Flavivirus (Crabtree et al, 2003; Sang et al, 2003; Cook et al, 2006; Zuo et al, 2014; McLean et al, 2015), Alphavirus (Nasar et al, 2012), Bunyavirus (Marklewitz et al, 2011, 2013; Auguste et al, 2014), Mesonivirus (Vasilakis et al, 2014; Wang et al, 2017), Almendravirus (Contreras et al, 2017), Reovirus (Attoui et al, 2005; Hermanns et al, 2014; Auguste et al, 2015; Harrison et al, 2016), and Negevirus (Vasilakis et al, 2013; Auguste et al, 2014; Kallies et al, 2014; Nabeshima et al, 2014; Carapeta et al, 2015; Kawakami et al, 2016; Fujita et al, 2017; O’Brien et al, 2017; Moraes et al, 2019; Wang et al, 2019; Zhou et al, 2019). Their high abundance, extensive viral taxa distribution and association to classical arboviruses warrants more in-depth research
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