Abstract
Strain Marseille‐P2341T, a nonmotile, nonspore‐forming, Gram‐stain‐positive anaerobic coccus, was isolated in the vaginal specimen of a patient with bacterial vaginosis using culturomics. Its growth occurred at temperatures ranging from 25 to 42°C, with pH between 6.5 and 8.5, and at NaCl concentrations lower than 5%. The major fatty acids were C18:1n9 (27.7%) and C16:0 (24.4%). Its genome is 1,671,491 bp long with 49.48 mol% of G+C content. It is composed of 1,501 genes: 1,446 were protein‐coding genes and 55 were RNAs. Strain Marseille‐P2341T shared 97.3% of 16S rRNA gene sequence similarity with Murdochiella asaccharolytica, the phylogenetically closest species. These results enabled the classification of strain Marseille‐P2341T as a new species of the genus Murdochiella for which we proposed the name Murdochiella vaginalis sp. nov. The type strain is strain Marseille‐P2341T (=DSM 102237, =CSUR P2341).
Highlights
Due to vaginal secretions and, sometimes, urine, the vagina is a humid biotope which constitutes a complex ecosystem colonized by several types of microorganisms (Pal et al, 2011)
In order to identify all bacteria present in the vagina and involved in this alteration, we studied normal vaginal flora and those from bacterial vaginosis using the concept of “microbial culturomics,” based on the multiplication of culture conditions with variations in temperature, media, pH, and atmospheric conditions, and rapid bacterial identification using matrix-assisted laser-desorption/ ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) (Lagier et al, 2012, 2015)
The 16S rRNA gene sequence of the strain exhibited 97.3% nucleotide sequence similarity with M. asaccharolytica, the phylogenetically-closest species with a validly published name (Figure 1). As this value was lower than 98.7%, the threshold recommended for delineating a new species (Kim et al, 2014; Stackebrandt & Ebers, 2006), strain Marseille-P2341T was classified as a new species named M. vaginalis (Table 1)
Summary
Due to vaginal secretions and, sometimes, urine, the vagina is a humid biotope which constitutes a complex ecosystem colonized by several types of microorganisms (Pal et al, 2011). In order to identify all bacteria (uncultured and fastidious) present in the vagina and involved in this alteration, we studied normal vaginal flora and those from bacterial vaginosis using the concept of “microbial culturomics,” based on the multiplication of culture conditions with variations in temperature, media, pH, and atmospheric conditions, and rapid bacterial identification using matrix-assisted laser-desorption/ ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) (Lagier et al, 2012, 2015) This microbial culturomics approach enabled us to isolate a new member of the Murdochiella genus that did not correspond to other species of this genus.
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