Characterization of 23 SNP markers for the chum salmon (Oncorhynchus keta) based on multiplex ISSR genotyping by sequencing (MIG-seq)

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Chum salmon (Oncorhynchus keta) is an important fisheries resources around the North Pacific. Although Japanese chum resources have declined dramatically in recent years, insufficient molecular markers have limited effective research, conservation, and management of this species. Multiplex ISSR genotyping by sequencing (MIG-seq) has the potential to identify neutral single-nucleotide polymorphism (SNP) markers. In this study, 23 novel SNP markers were developed for this species using MIG-seq. Observed and expected heterozygosities ranged from 0.050 to 0.600 and from 0.300 to 0.097, respectively. None of the developed SNPs deviated significantly from the Hardy-Weinberg equilibrium. Polymorphic information content ranged from 0.090 to 0.369. These findings suggest the availability of novel SNP markers for future studies on the population structure, conservation, and resource management of O. keta.