Abstract
Many plants accumulate proline in response to osmotic stress. Δ 1-pyrroline-5-carboxylate synthetase (P5CS) is the rate limiting enzyme in proline biosynthesis in plants. The promoter of an Arabidopsis P5CS gene was isolated and analyzed using a β-glucuronidase (GUS) reporter gene in transgenic plants. NaCl stress induced GUS activity to the maximum level within 3 h while dehydration continued to enhance the GUS activity up to 24 h. The P5CS gene promoter was found to contain two transcription start sites, and dehydration stimulated the transcription predominantly from the downstream site. Abscisic acid (ABA) failed to induce the P5CS gene expression although ABA is known to increase during water stress. These observations suggest that induction of the P5CS gene is a specific response of plants to salt and water stresses.
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