Abstract
Background Glarea lozoyensis is a filamentous fungus used for the industrial production of non-ribosomal peptide pneumocandin B0. In the scope of a whole genome sequencing the complete mitochondrial genome of the fungus has been assembled and annotated. It is the first one of the large polyphyletic Helotiaceae family. A phylogenetic analysis was performed based on conserved proteins of the oxidative phosphorylation system in mitochondrial genomes.ResultsThe total size of the mitochondrial genome is 45,038 bp. It contains the expected 14 genes coding for proteins related to oxidative phosphorylation,two rRNA genes, six hypothetical proteins, three intronic genes of which two are homing endonucleases and a ribosomal protein rps3. Additionally there is a set of 33 tRNA genes. All genes are located on the same strand. Phylogenetic analyses based on concatenated mitochondrial protein sequences confirmed that G. lozoyensis belongs to the order of Helotiales and that it is most closely related to Phialocephala subalpina. However, a comparison with the three other mitochondrial genomes known from Helotialean species revealed remarkable differences in size, gene content and sequence. Moreover, it was found that the gene order found in P. subalpina and Sclerotinia sclerotiorum is not conserved in G. lozoyensis. ConclusionThe arrangement of genes and other differences found between the mitochondrial genome of G. lozoyensis and those of other Helotiales indicates a broad genetic diversity within this large order. Further mitochondrial genomes are required in order to determine whether there is a continuous transition between the different forms of mitochondrial genomes or G. lozoyensis belongs to a distinct subgroup within Helotiales.
Highlights
The filamentous fungus Glarea lozoyensis (ATCC 20868) was originally isolated by plating filtrates of pond water near Madrid, Spain [1]
General Features The mitochondrial genome of G. lozoyensis ATCC 74030 comprises 45,038 bp, which is in the same range as several other mt genomes in Pezizomycotina (Table 1). 31.8% is covered with intergenic spacers of 3–1131 bp length and 15.04% with seven introns (Table 2)
We have identified the complete mt genome of G. lozoyensis on a scaffold obtained by whole-genome sequencing
Summary
The filamentous fungus Glarea lozoyensis (ATCC 20868) was originally isolated by plating filtrates of pond water near Madrid, Spain [1]. It came to be known as a producer of pneumocandins, non-ribosomal peptides with strong inhibitory effect on fungal glucan biosynthesis. In the scope of a whole genome sequencing the complete mitochondrial genome of the fungus has been assembled and annotated. It is the first one of the large polyphyletic Helotiaceae family. A phylogenetic analysis was performed based on conserved proteins of the oxidative phosphorylation system in mitochondrial genomes
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