Abstract
Municipal waste is one of the most hazardous components of developing countries. However, enzymes do provide an eco-friendly solution in this case. Amylase is an important enzyme in food, textile and pharmaceutical industry and can be used for bioconversion of waste. From the municipal solid waste we have isolated an amylase producing bacteria that can grow in the irritant municipal waste and help in their bio conversation. The bacteria were identified as Cronobacter sakazakii Jor52 (C2). The optimized media for maximum amylase production after 24 h of incubation, contains 2% starch, 0.6% peptone, 0.01% CaCl2, 0.05% KCl, 0.05% MgSO4 and 0.05% K2HPO4. The crude enzyme activity and stability study revealed that the amylase is stable within the pH 6 - 8 and temperature 30°C - 40°C and give maximum activity at 37°C at pH-8.
Highlights
We all are habituated with good-morning whistle of municipal “waste” collectors
From the municipal solid waste we have isolated an amylase producing bacteria that can grow in the irritant municipal waste and help in their bio conversation
We have used the municipal waste as a source of amylase producing bacteria so that the isolated strain will be able to tolerate the detrimental condition of municipal waste and produce enough amylase for their transformation
Summary
We all are habituated with good-morning whistle of municipal “waste” collectors. Diverse fungal strains are able to produce amylase both intracellularly and extracellularly depending upon the fermentation process [6,7,8] but bacteria are the preferred source because maximum amount of generated enzyme within a very short time period and due to its extracellular production attribute, which are easy to isolate [9,10,11]. Microbes can neither show proper growth nor enzyme production for waste bio-conversion in this adverse condition To outmaneuver this predicament, we have used the municipal waste as a source of amylase producing bacteria so that the isolated strain will be able to tolerate the detrimental condition of municipal waste and produce enough amylase for their transformation. The activity and stability patterns for the crude enzyme isolated from the bacterial strain were been reported
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