Abstract
The relationship between the sperm-coating antigens of rabbit seminal plasma origin and the characterization of the decapacitation factor was studied using a gar-gel diffusion immune-electrophoresis chromatography on Sephadix G-200 and polyacrylamide vertical gel electrophoresis. Spermatozoa incubated in the uterus for 11 hours yielded an 85% fertilization rate and hence were capacitated. Treatment of the capacitated spermatozoa with rabbit seminal plasma resulted in a 38% fertilization rate. It was concluded that a sperm-coating antigen of seminal plasma origin possessed biological activity for blocking fertilization. It was found using the above techniques that the sperm-coating antigen was a glycoprotein of approximately 170000 molecular weight migrated in an electric field similar to a serum slow beta-globulin and was still present in the seminal fluid of vasectomized males. The sperm-coating antigen was absent from inactive upper supernatant fluid fraction of seminal plasma after 4 hours of ultracentrifugation at 105000 g and was present in the active ultracentrifugal pellet.
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