Abstract
We examined 41 isolates of Penicillium spp. recovered from rotten fruits (including oranges, grapefruits, pears, lemons, strawberries, apples, loquats, prickly pears) and from air and surfaces of markets and packinghouses. Penicillium isolates were identified as P. expansum, P. italicum, P. digitatum, P. olsonii, P. chrysogenum or P. citrinum. Isolates were tested for sensitivity to commonly used postharvest fungicides, and pathogenicity on different potentially susceptible fruit hosts. Genetic characterization was performed with ITS4 and ITS5 primers that specifically identified Penicillium isolates by amplification of a 600-bp fragment, with PEF and PER primers used to identify P. expansum isolates by amplification of a 404-bp fragment, and with fluorescent amplified fragment length polymorphism analysis (fAFLP). Cluster analysis of fAFLP data divided the isolates into five well-separated P. italicum, P. digitatum, P. citrinum, P. chrysogenum and P. olsonii clusters, whereas P. expansum isolates were divided in three distinct clusters. Within all the eight clusters, isolates were well differentiated. Results obtained with fAFLP analysis confirmed the reliability of the method to characterize and identify strains at intraspecific level.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.