Abstract
Toll-interacting protein (Tollip) is a mediator involved in the TLRs signaling pathway which is critical for innate immune response. In the present study, a full-length Tollip cDNA was first cloned from common carp (CcTollip), which was 1284 bp in length, containing an open reading frame of 831 bp encoding a peptide of 276 amino acids. Multiple sequence alignment showed that the CcTollip shared the highest similarity with that of grass carp and zebrafish. Phylogenetically, the CcTollip clustered together well with their piscine family members. Quantitative real-time PCR analysis indicated that CcTollip was widely expressed in all tissues tested and showed up-regulation with challenges of Vibrio anguillarum and poly(I:C), suggesting that CcTollip was activated by V. anguillarum and poly(I:C). These data indicated that CcTollip might play an important role in immune response to bacterial and viral invasion.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-2293-3) contains supplementary material, which is available to authorized users.
Highlights
Common carp (Cyprinus carpio L.) is an important aquaculture fish species worldwide, but many bacterial and viral infections have recently become a big problem in common carp aquaculture industry
Cloning and sequence characterization of CcTollip The full-length cDNA of CcTollip was 1284 bp containing 92 bp 5′-untranslated region (UTR), an open reading frame (ORF) of 831 bp encoding a peptide of 276 amino acids and a 361 bp 3′-UTR
Conserved domain analysis with SMART program showed that CcTollip contained a Tom1-binding domain (TBD) (Tom1binding domain) extending from Met-1 to Arg-54, a conserved core domain 2 (C2) domain extending from Leu55 to Trp-151, and a coupling of ubiquitin to endoplasmic reticulum degradation (CUE) domain extending from Cys-231 to Ala-273 (Fig. 1)
Summary
Common carp (Cyprinus carpio L.) is an important aquaculture fish species worldwide, but many bacterial and viral infections have recently become a big problem in common carp aquaculture industry. The recognition of bacteria or virus by host cells is mediated by multiple pattern recognition receptors (PRRs), including Toll-like receptors (TLRs), NOD-like receptors (NLRs) and RIG-I-like receptors (RLRs). Among these PRRs, TLRs could recognize both bacteria and virus (Zhang and Gui 2012; Zhang et al 2014). Toll interacting protein (Tollip) is an important mediator in the innate immune responses induced by TLRs (Zhang and Ghosh 2002) It is first identified as a member of IL-1R pathway, which is presented in a complex with IRAK1 and inhibits IL-1-induced signaling by blocking IRAK1 phosphorylation (Burns et al 2000). Didierlaurent et al reported that Tollip regulated the magnitude and kinetics of IL-6 and TNF-α production upon stimulation with IL-1β and low doses or physiological doses of LPS (Didierlaurent et al 2006)
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