Abstract
Flow cytometry was used to identify mAbs that recognize conserved epitopes on hamster leukocyte differentiation molecules (hLDM) and also to characterize mAbs developed against hLDM. Initial screening of mAbs developed against LDMs in other species yielded mAbs specific for the major histocompatibility (MHC) II molecule, CD4 and CD18. Screening of sets of mAbs developed against hLDM yielded 22 new mAbs, including additional mAbs to MHC II molecules and mAbs that recognize LDMs expressed on all leukocytes, granulocytes, all lymphocytes, all T cells, a subset of T cells, or on all B cells. Based on comparison of the pattern of expression of LDMs expressed on all hamster leukocytes with the patterns of expression of known LDMs in other species, as detected by flow cytometry (FC), four mAbs are predicted to recognize CD11a, CD44, and CD45. Cross comparison of mAbs specific for a subset of hamster T cells with a cross reactive mAb known to recognize CD4 in mice and one recognising CD8 revealed they recognize CD4. The characterization of these mAbs expands opportunities to use hamsters as an additional model species to investigate the mechanisms of immunopathogenesis of infectious diseases.
Highlights
The golden or Syrian hamster (Mesocricetus auratus) is used in biomedical research as a model for human and other animal diseases where the mouse is not appropriate
When using the cross reactive monoclonal antibodies (mAbs) that recognize epitopes conserved on hamster CD4 and CD8 T cells, a fluorescein conjugated goat mouse-absorbed anti-rat IgG second step was used for rat GK1.5 IgG2b mAb
Flow cytometry was used to screen for mAbs that recognize conserved epitopes expressed on hamster leukocyte differentiation molecules (hLDM) (Saalmüller et al, 2005)
Summary
The golden or Syrian hamster (Mesocricetus auratus) is used in biomedical research as a model for human and other animal diseases where the mouse is not appropriate. Hamsters offer an opportunity for adoptive cell transfer experiments to explore pathogenesis, as they are highly inbred (Campbell et al, 1996). This may be attributable to the current lineage being derived from three siblings caught in 1930 limiting genetic heterogeneity and functionality (Phillips et al, 1981). The usefulness of the hamster as a small animal model for biomedical research has been constrained by a lack of immunological reagents to detect LDM differentially expressed on lymphoid cell subsets. These mAbs are available to the research community for further detailed characterisation
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