Abstract
The solute carrier family 11 member A1 (SLC11A1) gene has been associated with natural resistance to intracellular pathogens such as Brucella, Salmonella, Leishmania and Mycobacterium in several species including bovine and plays a critical role in elimination of pathogens by generating hydroxyl free radicals. The objective of the present study was to investigate the polymorphism in exon 9 of SLC11A1 gene in Vechur cattle, one of the dwarf cattle of India which is known for its disease resistance. A 198 bp fragment containing exon 9 of the gene was amplified by polymerase chain reaction (PCR). The amplicons upon single strand conformation polymorphism (SSCP) analysis revealed two different banding patterns. A novel non synonymous SNP (g.46C>T) with predominance of CC genotype was also detected in Vechur cattle. These results suggest that there exists a considerable genetic variation at SLC11A1 locus and further association studies may help in development of a PCR based genotyping assay to select cattle with better immunity to intracellular pathogens.
Highlights
Dairy industry is affected by various infectious diseases such as Brucellosis, Paratuberculosis, Salmonellosis caused by various intracellular pathogens
solute carrier family 11 member A1 (SLC11A1) gene formerly known as Natural Resistance Associated Macrophage Protein 1 (NRAMP1) gene encodes a protein with 12 transmembrane domains which is involved in the transport of divalent cation such as Fe2+, Mn2+, Zn2+, Mg2+ and Co2+ ions (Vidal et al, 1996; Goswami et al, 2001)
SNP in exon 4 and intron 4 were reported by Cheng et al (2015), suggested that exon 4 polymorphism was corresponding to a non synonymous mutation, which was associated with tuberculosis in Holstein cattle
Summary
Dairy industry is affected by various infectious diseases such as Brucellosis, Paratuberculosis, Salmonellosis caused by various intracellular pathogens. These infections has a significant economic impact and causes substantial financial losses every year. Due to a more efficient host immunological defence, native breeds are found to be resistant against various pathogens. These resistance may be associated with one or more host genes. SLC11A1 gene formerly known as Natural Resistance Associated Macrophage Protein 1 (NRAMP1) gene encodes a protein with 12 transmembrane domains which is involved in the transport of divalent cation such as Fe2+, Mn2+, Zn2+, Mg2+ and Co2+ ions (Vidal et al, 1996; Goswami et al, 2001). SLC11A1 has many pleiotropic effects on macrophage function that includes enhanced tumor necrosis factor-α, interleukin- 1β, inducible nitric oxide synthase and MHC class II expression which are important in resistance to intra cellular pathogens such as Mycobacterium tuberculosis (Awomoyi, 2007)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
