Abstract
SummaryA virus, isolated from the nutrient feeding solution of a bell pepper culture in a glasshouse, was characterised as a new serotype of tobacco necrosis necrovirus (TNV) and named TNV‐nft. The virus was monopartite and contained a single RNA species and a single coat protein with molecular weights of 1.45± 106 and 2.9 ± 104 daltons, respectively. The particle had an apparent sedimentation coefficient of 110 S and a density of 1.37 g/cm3 in cesium chloride. In decoration tests antiserum prepared against TNV‐nft reacted most strongly with the TNV strains Kassanis A and B, but at lower dilutions it also reacted with all other TNV‐serotypes available. In Ouchterlony tests spur formation occurred with TNV strains Kassanis A and B, indicating that the TNV‐nft strain was serologically distinct. The isolate was not associated with a satellite virus. Cloned cDNA prepared against viral RNA reacted exclusively with the viral RNA band in Northern blots.
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