Abstract

In this chapter I discuss polarization microscopy and how it can be used to provide contrast to transparent and would-be invisible birefringent specimens, and to determine the orientation of birefringent molecules such as cellulose, starch, and DNA, in the specimen. I discuss what polarized light is, how to use an analyzer to test for polarized light, how to produce polarized light, the interference of polarized light, how birefringent specimens turn linear polarized light into elliptically polarized light, the production of color in birefringent specimens, how to make a polarized light microscope, the molecular basis of birefringence, and how to use compensators to determine the sign and magnitude of birefringence.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.